#N207) LAL Reagent Water (Lonza cat. The cytotoxicity of the CDDP loaded CHAMs were done by treating the FaDu cells with DMEM supplemented with 0. . The plate was read on a SpectraMax 340 PC plate reader (Molecular Devices, Sunnyvale, CA) at 450 nm, with reference at 650 nm. Gerritsma,B. The absorbance is determined at 620 nm on a SpectraMax 340 PC 384. The first time you replace an OEM lamp you must use the N-34-MLA. What is h the bandwidth for SpectraMax® plate readers and how do we determine it? The instrument bandwidth for the reader is expressed as half-bandwidth, meaning the full bandwidth at 1/2 the transmission peak for the selected wavelength. MCE has not independently confirmed the accuracy of these methods. The reaction mixture was incubated for 5 min. Lactate dehydrogenase (LDH) release assay Cytotoxicity was determined by measuring the release of LDH. When reading optical density at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. Each experiment was performed in duplicate and repeated at. Page: 1/6. Microplate Reader Shelf. After washing, the dye was eluted with 3% HCl and the absorbance measured at 665 nm with a SpectraMax 340 spectrophotometer (Molecular Devices, Sunnyvale, CA). FACS analysis. A. control. The absorbance was measured at 340 nm for 2 min at a 15 s time interval. 306 plate air cal. Despite the relevance of adaptive immunity against equine pathogens antigen-specific T cell responses of horses are not well characterized and the lack of insight into T cell responses hampers the understanding of the pathogeneses of important diseases. SpectraMAX 340 microtiter plate reader. 5 mL sample or standard solution was well mixed with 0. Not. The cell death was calculated as follows:Absorbance was monitored at 450 nm (SpectraMax 340). 20. Product Keyword. Novak, Nathan J. Data are expressed as percent of maximum binding for each protein. A. 2. Samples were analyzed in triplicates. The VersaMax and SpectraMax 190 read 96-well microplates. 7. ). 260 280 300 320 340 360 Instrument: Molecular Devices SpectraMax 250 Instrument: LAMBDA 19/UV/VIS/NIR O. 315 can't find zero order. Afterwards, absorbance at 595 nm was measured with spectrophotometer (SpectraMax 340, Molecular Devices, USA). 1 mg mL −1 CDDP loaded A-CHAM, B-CHAM, C-CHAM or D-CHAM. 3. 05% at 340 nm Photometric stabilization Instantaneous Photometric drift None—continuous referencing of monochromatic output Calibration Automatic before first kinetic read and. Calculating % of total mediator release in RBL assay. 5 mL of 1 M acetate buffer (pH 4. Incubate 30 min at room temperature, and then read microplates at 405 nm using SpectraMax 340 PC plate reader (Molecular Devices) (see Note 8). 1 mL of acetylacetone. Time-resolved fluorescence was measured on the SpectraMax i3x and SpectraMax iD5 readers using preconfigured protocols in SoftMax® Pro Software (see Tables 2 and 3 for instrument-specific settings). This tunable visible absorbance reader is for 96- and 384-well microplates and acquires data in endpoint, kinetic, and spectral scan modes using wavelengths from 340-850 nm, tunable in 1. The reaction mixture was incubated for 5 min. When reading optical density at wavelengths below 340 nm, special UVtransparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. Both readers feature an 8-channel read head for fast reads and. California Lightworks uses only the highest quality LEDs from Osram. 1. Buffer (100 mmol L –1acetate buffer, pH 5. Reagena Poc Puumala® was used for detection of IgM antibody against PUUV in NE patient sera at the Department of Clinical Virology,. PerkinElmer offers a variety of Microplates For Absorbance and Colorimetric Assays. 4. ), which was proportional to the number of damaged cells. SpectraMax Injector Cartridge with SmartInject™ Technology. ). Binding of capture antibodies to polystyrene beads and biotinylation. Molecular Devices SpectraMax 340 PC 384 Microplate Reader. 4. TRF-based with 340/80 nm EX and 616/10 nm EM 0200-7027 EX range: 340/80 nm EM range: 616/10 nm High fg levels of Streptavidin High fg levels of Streptavidin 2 AlphaScreen AlphaScreen and AlphaLisa detection using 1 W 680 nm EX laser diode and a 570 nm (100) EM filter Pick best speed, sensitivity, and price for your needs Table 1. The absorbance is determined at 620 nm on a SpectraMax 340 PC 384. Subsequently, the oxidative burst was initiated by the addition of PMA (final concentration 100 nM) and the cells incubated for 120 min at 37°C. TheSpectraMax®340PC384,SpectraMax®190,andVersaMax™microplate spectrophotometersproviderapidandsensitivemeasurementsofavarietyofanalytesacross. 5mM), and changes in OD 405 nm. Emission. or microplate reader (e. Narrow bandwidths deliver increased measurement accuracy and linearity over a wide range of assays. Products : SpectraMax Plus 384 reader,. g. Following cell culture under normoxic or hypoxic con-ditions, culture medium samples were obtained on completing the incubation period to measure atrial natriuretic peptide. The Molecular Devices SpectraMax 340PC384 Microplate Reader can run both standard spectrophotometer and microplate reader applications on the same instrument. Samples containing 25 μg of protein were separated on 12. 3T3 cells were cultured in DMEM supplemented with 10% FCS and 1% antibiotics (10,000 units/ml penicillin and 10,000 μg/ml streptomycin in 0. ELISAs were read with a Spectramax 340 from Molecular Devices (Sunnyvale, CA, USA). 8. Permeability of the monolayer was verified by adding 1% Evans Blue dye (Thermo Fisher Scientific, Inc. Manual: SoftMaxPro User Guide Manufacturer: Molecular Devices Location: SOM-E536 The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. Percent radical scavenging activity was determined by comparison with a DMSO containing control (shown in Table 3). SPECTRAmax 340PC Microplate Spectrophotometer Operator’s Manual 1-3 Introduction General Overview The SPECTRAmax ® 340PC incorporates a holographic grating. 2 g/L solution; dilute 1:10 in HBSS. 8 pin mini DIN (Spectramax 340) to 9D (PC) serial data transfer cable. The SPECTRAmax 340PC can mea-The extracted stain was then transferred to a 96-well plate, and the absorbance at 562 nm was measured using a SpectraMax 340 plate reader/spectrophotometer(Molecular Devices Corp. Growth was monitored at 30°C every 6 min for 8 to 12 h at 660 nm using a multiscan photometer (spectraMax 340; Molecular Devices). Cell viability wasDescription Microplates The SpectraMax Multi-Mode Microplate Reader can accommodate SBS- standard 6-well to 384-well microplates and strip wells. For more sample throughput, the system canThe optical density (OD) at the wavelength of 450 nm was measured with a spectrometer (SpectraMax 340, Sunnyvale, CA, USA). Enzyme activities at high concentration were analyzed using a SpectraMAX 340 plate reader (Molecular Devices) at 380 nm in the kinetic mode at 37°C. 6 g sodium acetate trihydrate and 2. TheSpectraMax®Plus384addstheabilitytoreadcuvettes. SPECTRAmax 340PC 384 Microplate Spectrophotometer Operator’s Manual vii Conventions Used in this Manual The names of keys that appear on the SPECTRAmax PLUS 384 control panel are shown in boxed Helvetica type. Not all manufacturers' microplates are the same with regard to design, materials, orAbsorbance at 405 nm (Abs 405) was obtained for both plates before (background) and after 1-h incubation at 37°C using a microplate reader (SpectraMax 340 PC384, Molecular Devices). SpectraMax Multi-Mode Microplate Readers. After 72 h, 20 μl of 5 mg/ml MTT solution was added to each well and plates were incubated for 3 h at 37°C. Cross sections of mouse skin was prepared and stained as previously described . 0, MSD) or Spectramax 340 (Molecular Devices). Absorbance was measured at 450 nm by using a SpectraMax 340 microplate spectrophotometer (Molecular Device). Since the formation of NADPH is stoichiometrically related to the oxidation of glucose-6-phosphate, measurements for glucose + fructose can be determined by the absorbance of NADPH at 340 nm. 5 mL of 1 M acetate buffer (pH 4. SPECTRAmax 340PC Microplate Spectrophotometer Operator’s Manual 1-3 Introduction General Overview The SPECTRAmax ® 340PC incorporates a holographic grating monochromator which allows you to specify a precise wavelength, from 340 nm to 850 nm, for the absorbance maximum of your sample. Molecular Devices ThermoMax is a high-efficiency, temperature-regulated, 96-well microplate reader. The cell growth was determined by measuring the optical density at 600 nm using a spectrophotometer (SpectraMax 340, Molecular Devices, USA). When reading optical density at wavelengths below 340 nm, special UVtransparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. IMAP ® Technology from Molecular Devices enables rapid, homogeneous, and non-radioactive assay of kinases, phosphatases, and phosphodiesterases and is suited for both assay development and high-throughput screening. The Analyst®, FlexStation® and SpectraMax® M5/M5 e from Molecular Devices have received the LanthaScreen® Certified designation from Life Technologies which ensures that these readers are validated to strict standards in instrument setup and assay performance. 5 mL sample or standard solution was well mixed with 0. Liquid handling for random screening was carried out with a Biomek FX from Beckman Coulter (Fullerton, CA, USA) and HYTRA. The inhibition percentage was determined by linear regression through ranges of 20 to 30. • TRF-based with 340/80 nm EX and 616/10 nm EM 0200-7027: EX range: 340/80 nm EM range: 616/10 nm: High fg levels of Streptavidin: High. SpectraMax Multi-Mode Microplate Readers. Molecular Devices SpectraMax 340 PC 384 Microplate Reader. #PLUS 384) MethodsSpectraMax 340 PC, Molecular Devices, USA was used as a 96-well microplate reader to test bioactivities. The results of ELISA optical density (OD) values in duplicates were averaged for the following analyses. WST regents of 10% were added to each well, and absorbance was determined at 450 nm using an ELISA reader (SpectraMax® 340, Molecular Device Co. VersaMax reads 96-well and strip-well plates. 0112-0126B 8 Chapter1:Introduction TheSpectraMax®340PC384,SpectraMax®190,andVersaMax™microplate. Lactate Dehydrogenase (LDH) Release. 5 stars - 1602 reviewsThis assay typically develops in 1. Turn on the Spectramax 340PC spectrophotometer using the switch located next to the plug in the back on the right hand side (as you face the spec). The optical system of the SpectraMax 340PC384 Microplate Reader is built around a monochromator, which allows for selection of up to six wavelengths at a time for. TDP-43 stress granular cells were treated with 70% ice-cold. ): 8. automated SpectraMAX 340 (Molecular Devices, Sunnyvale, CA, USA) microtiter plate reader. The adapter is. SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A SpectraMax Plus 384 SpectraMax 190 SpectraMax 340PC 384 VersaMax. TGFα release was calculated as described in the Results. 2. Acceptor: 665nm. SpectraMax 340PC 384 and SpectraMax Plus 384 read both 96-and 384-well microplates. After 24 h, 48 h, 72 h or 96 h, cell viability was measured by CellTiter 96® AQueous One Solution cell proliferation assay (Promega, Madison, WI, USA). Create custom fluorescence assays without having to purchase expensive filter sets. The SpectraMax® ABS and ABS Plus absorbance microplate readers provide the flexibility, sensitivity, and convenience for a wide range of assays such as ELISAs, microbial growth, and protein quantitation. Scanning was done at 600 nm, using a Molecular Devices SpectraMax 340 plate reader. For more sample throughput, the system can The optical absorption was measured at 450 nm for BUN and 490 nm for creatinine with a spectrometer (SpectraMax 340). 304 signal level saturation. Background: Ketamine can act as a multifunctional neuroprotective agent by inhibiting oxidative stress, cellular dysfunction, and apoptosis. SpectraMax Gemini EM & FLEXstation (bottom read) 1. using SpectraMax i3 (Molecular devices, San Jose, USA). ac. 0% and ± 0. (1988) and recorded at 412 nm. 3. 5. The amount of liberated pNA product during incubations was determined using a standard curve obtained at 410 nm for various concentrations of a pNA stock solution in the SpectraMax 340 microplate reader. Typically the system can detect absorbance, luminescence, fluorescence, and even make more specialized fluorescence measurements like time-resolved fluorescence (TRF) and fluorescence polarization (FP). The SpectraMax® Plus 384 Microplate Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. Immunofluorescence and Aggregation Analysis. LBA Formats for Pharmacokinetic AssaysAfter treatment, 10 μl of CCK-8 was added to each well, and the incubation was continued at 37°C for 2 h in the dark. 0112-0126B 8 Chapter1:Introduction TheSpectraMax®340PC384,SpectraMax®190,andVersaMax™microplate. All ELISA plates were analyzed using a SpectraMax 340 PC microplate reader (Molecular Devices). This Molecular Devices SPECTRAmax 340PC incorporates a holographic grating monochromator which allows you to specify a precise wavelength, from 340 nm to 850 nm, for the absorbance maximum of your sample. Immunofluorescence and Aggregation Analysis. Values are expressed as the mean s ± SD of 3 separate experiments, each performed in triplicate. Changes in cell viability were reflected by changes in optical density detected at 450 nm, which was measured using a spectrophotometer microplate reader (SpectraMax 340; Molecular Devices, Sunnyvale, CA, USA). The reaction was then stopped by adding 50 µL of 1 M sulfuric acid to each well. SpectraMax 340 (Serial number starting with M) SpectraMax 340PC (Serial number starting with L) SpectraMax Plus (Serial number starting with P) Tmax Also refer. , San Jose, CA) using Softmax Pro 7 software. Percent Transmission Absorbance Competitor 0. 1 mL of culture media with 10% FBS. 0 0 The indicated microplates were scanned form 200 nm to 800 nm. SpectraMax 340PC 384 and SpectraMax Plus 384 read both 96-and 384-well microplates. measurements were performed on a SPECTRAmax-340 plate reader (Molecular Devices Inc. Description Control Panel Cuvette Chamber Back Panel Microplate Drawer The Control Panel TEMP on/off TEMP Figure 1. using SpectraMax i3 (Molecular devices, San Jose, USA). 6, 2 mmol/l DTT, 10 mmol/l MnCl 2 and 10 mmol/l pNPP; the PP2Cα enzyme concentration was 400nmol/l. #N207) LAL Reagent Water (Lonza cat. SpectraMax QuickDrop Micro-Volume Spectrophotometer. The values of IC 50 represent concentrations of compounds toA microplate reader (SpectraMax M5; Molecular Devices, Sunnyvale, CA) was used to measure the absorbance at 340 nm for the collected BPW (6) Stabilization protects islet integrity during respirometry in the Oroboros Oxygraph-2K analyzer Islets December 31, 2022 Justin J. The SpectraMax ABS Plus, can accommodate standard 96-well plates and 384-well plates. Crowder, Ziqian Zeng, Alissa N. Although it has been proven to be effective in various neurologic disorders, the mechanism of the treatment of traumatic brain injury (TBI) is not fully understood. 1% N-1-naphtylethylenediamine dihydrochloride, 2. 340/80 nm excitation with 616/10 nm emission range Optimized for use with ScanLater Western Blot Detection cartridge for the SpectraMax® i3, i3x, and Paradigm® Multi-Mode Readers Optimized for use with the enhanced TRF detection module for the SpectraMax iD5 Multi-Mode Microplate ReaderThe optical density value was measured at absorbance 450 nm by a microplate reader (SpectraMAX 340, molecular devices, USA) for cell viability. Awareness Technology, Inc. P. Plasminogen was added to the fibrinogen before addition of thrombin and either tissue plasminogen activator (tPA) or urokinase plasminogen activator in the. Cet appareil ISM est confomre à la norme NMB-001 du Canada. The Analyst®, FlexStation® and SpectraMax® M5/M5 e from Molecular Devices have received the LanthaScreen® Certified designation from Life Technologies which ensures that these readers are validated to strict standards in instrument setup and assay performance. Oxidative stress induction by H 2 O 2 and intracellular ROS analysis in L-929 cells. The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. Unit has several areas of damaged plastic. The Molecular Devices SpectraMax 340PC384 Microplate Reader can run both standard spectrophotometer and microplate reader applications on the same instrument. 003 OD Stray light ≤ 0. When reading at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. WST regents of 10% were added to each well, and absorbance was determined at 450 nm using an ELISA reader (SpectraMax® 340, Molecular Device Co. 3 mm. The SpectraMax ABS Plus, can accommodate standard 96-well plates and 384-well plates. The absorbances were being measured at 515 nm. After then, 10% of ammonium hydroxide solution was added to neutralize the aqueous solution of the ARS extracts, and the absorbance values were measured using a SpectraMax® 340 plate reader at 405 nm. Changes in OD 405 nm were followed on the SpectraMAX 340 plate reader. , Sunnyvale, CA). 2. They are for reference only. #25-340) Pyrogen-free Dilution Tubes (Lonza cat. 2 M DEEMM were mixed in a microtube,. Near bottom. Upgradeable modules are also available including western blot, cell imaging, and fast. The assay was carried out in a 96-well plate in a final volume of 100 µl containing 50 mmol/l Tris-HCl, pH 7. The absorbance at 450 nm was determined using a microplate reader (spectraMAX 340). Although all samples initially were analyzed at the same volume, the volume of sample. Photometric Range: 0. The ratio of red to green fluorescence intensity was calculated. This is a used Molecular Devices SpectraMax 340PC Microplate Spectrophotometer. These lab workhorses are used in drug discovery, research, bioassay validation, and. 0200-7027. Molecular Device M2e. Perkin Elmer Wallac Victor2 1420 Multilabel Counter $1,500. 340 nm. When reading at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates and cuvettes that allow transmission of the. , Fremont, CA, USA). Italic and boldface type are used for emphasis. 5. To. Donor: 620 nm. The relative cell viability was determined as the percentage of the absorbance value in the cells to the absorbance value of a control group. Includes: Computer Loaded with Softmax Pro 5. 303 unable to cal dark current. SpectraMax Multi-Mode Microplate Readers. 8 pin mini DIN (Spectramax 340) to 9D (PC) serial data transfer cable. CD154 is an established indicator of antigen-reactive T helper cells in other species, but has not been characterized in horses. Bioz Stars score: 86/100, based on 1 PubMed citations. Each SpectraMax® 340 PC 384 Microplate Reader has an. Details. The activity of generated thrombin was measured by the amidolysis of its substrate S-2238 and theThe purple formazan crystals formed were dissolved by adding 100 μL of DMSO to each well and absorbance was read at 570 nm in a spectrophotometer [SpectraMax 340]. Afterwards, absorbance at 595 nm was measured with spectrophotometer (SpectraMax 340, Molecular Devices, USA). 4) was followed according to Vanacker et al. The SpectraMax i3x reader provides the flexibility to add novel detection capabilities without the need for service engineers or costly downtime—it grows with you as your research areas expand. 3 0. The growth. The SPECTRAmax 340PC can measure. A 0. Molecular Devices VersaMax Absorbance Microplate Reader, 340-850 nm, Molecular Devices (CAT#: STEM-SPO-0005-YJL) Labsystems Fluoroskan II Fluorescent Microplate Reader, 320 – 700 nm. When reading at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. SpectraMax 340PC384 reads 96-well plates and 384-well plates and strip-well plates. Stat Fax 4700 Microstrip Reader. Exhibit with us {{>currencyLabel}} Back. With SOFTmax PRO, the contents of the wells in a microplate can be mixed automatically by shaking before each read cycle, making it possible to. Molecular Devices SpectraMax 340 PC 384 Microplate Reader. The wavelength and scanning ranges are from 340–850 nm in 1 nm increments. MOLECULAR DEVICE M5. The SpectraMax 340PC 384 and SpectraMax Plus 384 read both 96-and 384-well microplates. Reader is the cornerstone of a complete laboratory solution to help you expand the boundaries of your research capabilities. 0 OD. Bacterial growth was measured after every 45 min by recording the absorbance at 595 nm using a SpectraMax 340 spectrophotometer (Molecular Devices, Sunnyvale, CA, USA). TGFα release was calculated as described in the Results. CSF-1, IL-34 and calprotectin levels were analysed by ELISA according to instructions provided by the manufacturer (R&D Systems, Minneapolis, Minnesota, USA). Heme was preincubated for 5 min with A when added before the metals. Excitation 1 340 nm Emission 1 520 nm Cut off for emission 1 495 nm Excitation 2 340 nm Emission 2 620 nm Cut off for emission 2 570 nm Sensitivity Readings 50 to 100 PMT Optimal Column Wavelenght Column Priority . 5. A 340 μL of 50 mM borate buffer (pH 9), 240 μL methanol, 10 μL standard solution or sample, and 20 μL of 0. 00 USD. using a Molecular Dynamics Model SpectraMAX 340 PC microplate reader. Molecular Devices Corporation 1311. Clotting times (mean ± SD) for rSimukunin and rSV. SpectraMax M2, while top and bottom reads are possible on the SpectraMax M2 e. The SpectraMax ABS Plus reader was heated to 37ºC prior to setting up the. well, and strip-well microplates. The SpectraMax ABS/ABS Plus Microplate Readers provide fast absorbance detection without the use of filters with monochromator-based wavelength selection for visible and. Molecular Devices. Continuous kinetic monitoring of enzyme activity was performed on SPECTRAmax 340 or Flexstation 2-384 microplate reader (Molecular Devices, Sunnyvale, CA, USA) and controlled by the Softmax software. 5. The supernatants were mixed with an LDH assay reagent, which was purchased from Takara Bio Inc. SpectraMax Multi-Mode Microplate Readers. Male 8 pin mini Din to Female 9D. Details. Following incubation for 30 min at room temperature, the reaction was quenched by addition of 100 μl stop solution (2 N H 2 SO 4) and absorbance was read using microplate reader (SpectraMax 340; Molecular Devices Co. After the addition of sPLA2 (20 μ g), the reaction mixture was incubated for 40 min at 37°C, and the absorbance was read at 10 min intervals. Molecular Devices. Tunable for filterless flexibility. 315 can't find zero order. SpectraMax Multi-Mode Microplate Readers. Microscopy. When reading optical density at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. We selected the response at t=10 h for statistical analysis, because at that time point the response variation was largest. Each experiment was performed in duplicate and repeated at. . Dissolve 13. Absorbance was then measured at 540 nm (isosbestic point of cytochrome c) and 550 nm (SpectraMax 340; Molecular Devices, Sunnyvale, CA, USA). 7 macrophages were obtained from ATCC (Manassas, VA). edu Contact Phone: 216-368-8962. The SpectraMax Vertical 1000 uses only the highest-efficacy ams-Osram LEDs with efficiencies up to 4. 4- to 5-weeks old female BALB/c mice were purchased from Charles River Laboratories (QC, Canada). Subsequent replacements require only the lamp (N-34-LB) be replaced. Alkaline phosphatase (ALP) staining assay ALP activity was detected using a SensoLyteVR pNPP Alkaline Phosphatase Assay Kit (AnaSpec, Inc. #N207) LAL Reagent Water (Lonza cat. Top and bottom reads are available for fluorescence, time-resolved fluorescence and luminescence detection. , Sunnyvale, CA, USA) at 595 nm. 5. , Sunnyvale, CA). ThermoMax performs automatic mixing before and between readings to ensure reliable and precise results. SoftMax Pro. 5 - 2. Centrifuge – Eppendorf 5417C with rotor FA 45–30–11. After washing, the dye was eluted with 3% HCl and the absorbance measured at 665 nm with a SpectraMax 340 spectrophotometer (Molecular Devices, Sunnyvale, CA). 340 nm / 70 nm. 2 were considered negative for IgM antibody. Briefly, ear or dorsal skin was dissected and fixed with 10% phosphate buffered formalin for 24–72 h. With SOFTmax PRO, the contents of the wells in a microplate can be mixed SpectraMax M2, while top and bottom reads are possible on the SpectraMax M2 e. TGFα release was calculated as described in the Results. 04. Description 1. SpectraMax 190 reads 96-well and strip-well plates. Thin layer chromatography (TLC) was. The concentration of TNFα in the experimental samples was calculated from a mouse TNFα standard curve of 15–2000 pg/ml. Each experiment was performed in duplicate. The ability of PTK787/ZK 222584 to inhibit angiogenesis was tested in an in vitro model of capillary sprout formation (64) . When reading optical density at wavelengths below 340 nm, special UVtransparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. Isolation of RNA and RT-PCR. SpectraMax Multi-Mode Microplate Readers. Stat Fax 3200 Microplate Reader. This is equivalent to having 511 built-in filters,Results: A total of 64% of the subjects reported improved quality of sleep during treatment with naloxone at a dose of 40 ng per 24 hours as compared with 9% with sham treatment (P=0. 5. Temperature Range: Ambient + 4 °C up to 45 °C. Or scan up to 384 wells at any time to determine the best assay conditions. Lactate dehydrogenase (LDH) release assay Cytotoxicity was determined by measuring the release of LDH. 96-well clear LAL Reagent Grade™ Multi-well Plates (Lonza cat. #ABS PLUS) Methods. Availablility: By Availability Molecular Devices SpectraMAX 340 Microplate Reader. The optical system of the SpectraMax 340PC384 Microplate Reader is built around a monochromator, which allows for selection of up to six wavelengths at a time for absorbance detection in the visible wavelength range (340 nm - 850 nm). With SOFTmax PRO, the contents of the wells in a microplate can be mixed automatically by shaking before each read cycle, making it possible to. 313 reference gain check fail. As discussed below, dilution of the culture was required because cell density is not proportional to A 600 when the values are above ;0. 2. Fully tested with Warranty. 0. IMAP assays are based on binding of phosphate to immobilized metal coordination complexes on nanoparticles. SpectraMax i3/i3x and Paradigm readers can be equipped with the HTRF detection cartridge. MSD read buffer and TMB one component HRP substrate are from MSD and SurModics (Eden Prairile, MN), respectively. Measurements of OD at 540 nm were then performed in a microtiter plate reader (SpectraMax 340 microplate reader; Molecular Devices Corp. Absorbance was read at 405 nm on a microplate reader (SPECTRAmax 340 PC). SpectraMax M2, while top and bottom reads are possible on the SpectraMax M2 e. On-site Repair Service available nationwide for Molecular Devices Spectramax 340 Plate Reader's. Our absorbance plate readers feature our PathCheck Sensor technology and. Microplate Readers. Background: Ketamine can act as a multifunctional neuroprotective agent by inhibiting oxidative stress, cellular dysfunction, and apoptosis. The top- and bottom-reading Gemini EM Microplate Reader with dual monochromators lets users determine the optimal excitation and emission settings for creative new fluorescence. Subsequently, the oxidative burst was initiated by the addition of PMA (final concentration 100 nM) and the cells incubated for 120 min at 37°C. Other technologies such as imaging. High fg levels of Streptavidin High fg levels of Streptavidin Exceeds Cisbio certification requirements TRF-based with 340/80 nm EX and 616/10 nm EM AlphaScreen AlphaScreen and AlphaLisa detection using 1 W 680 nm. When reading at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. Bertin Precellys Evolution Touch Homogenizer. This Molecular Devices SPECTRAmax 340PC incorporates a holographic grating monochromator which allows you to specify a precise wavelength, from 340 nm to 850 nm, for the absorbance maximum of your sample. Products : SpectraMax Paradigm reader, SpectraMax Plus 384 reader, SpectraMax 250 (Obsolete), SpectraMax L reader, SpectraMax 340PC 384 (Obsolete), Gemini EM reader, Gemini XPS reader, SpectraMax 340 (Obsolete). The Gemini EM Reader with dual monochromators allows researchers to try new and novel dyes without having toThe absorbance was determined to be 450 nm using an ELISA reader (SpectraMax® 340, Molecular Device Co. Number of flashes. The area under the curve (AUC) for the comparison of the growth rates was determined using. Read one sample or up to 384 in a single plate read using any standard cuvette, or 96- or 384-well microplate. , Sunnyvale CA). a SpectraMAX 340VR microplate reader (Molecular Devices, Sunnyvale, CA, USA). Glutathione reductase (GR) activity was measured, according to Smith et al. 05% at 340 nm Photometric stabilization Instantaneous Photometric drift None—continuous referencing of monochromatic output Calibration Automatic before first kinetic read and. Read up to 6 wavelengths in endpoint or kinetic mode for 96- and 384-well plates, and perform spectral scans to determine. Optical density was measured at 570 nm using a microplate reader (SpectraMAX 340, Molecular Devices Co. , Sunnyvale, CA) after incubation of solution mixture at 37 °C for 2 h 30 min in the dark. Capable of detecting DNA down to 25 ng per well, this model gives users the same sensitivity as conventional systems to produce accurate nucleic acid measurements. The plates were incubated for 4 additional hours at 37°C. Operators can conveniently select up to 6 wavelengths at a time for absorbance detection in the visible wavelength range 340 nm - 850 nm. Hydrogen peroxide (H 2 O 2) was used to establish an intracellular oxidative. SpectraMAX 340. Values were determined according to a standard curve (log/log curve-fit). 0 Micromoles per Joule, ensuring the highest light output using the least amount of electricity. The optical density of each sample was analyzed at 450 nm with a reference reading at 630 nm using a SpectraMax 340 absorbance plate reader (Molecular Devices, Union City, CA). The SpectraMax M2 is a dual-monochromator, multi-detection microplate reader with a dual-mode cuvette port and 96 or 384 microplate reading capability. The plate was covered and incubated for one hour at room temperature. Please see photos. plates can be used in the SPECTRAmax PLUS 384. the SpectraMax iD3 is a fantastic plate reader for a. On 1, 7, 14, and 21 days, the cells were washed with DPBS twice and incubated with CCK-8 solution for 2 h in the dark at 37 °C. FlexStation® 3 Multi-Mode Microplate Reader. LAL Reagent Grade ™ Multi-well Plates (Lonza cat. ScanLater Western Blot Detection Cartridge. The drawer will open and it may close, if is doesn't, close it using the DRAWER button found on the Spectramax control panel (pictured in purple on. using SpectraMax i3 (Molecular devices, San Jose, USA). SpectraMax 250 With Computer SoftwareSpectraMax 340PC384 Microplate Reader A versatile 96- and 384-well format absorbance reader let the monochromator tune to that exact wavelength. SpectraMax® Gemini™ EM Microplate Reader, Molecular Devices. The SpectraMax® Plus 384 Microplate Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. These models can no longer be repaired. SpectraMax 340PC 384 and SpectraMax Plus 384 read both 96-and 384-well microplates. 0112-0115J 9 Chapter1:Introduction TheSpectraMax®M3Multi-ModeMicroplateReader,SpectraMax®M4Multi-ModeMicroplate Reader,SpectraMax®M5Multi-ModeMicroplateReader,andSpectraMax®M5eMulti-Mode Opening the Drawer Manually on page The SpectraMax ABS can accommodate standard 96-well plates. 10× trypsin 0. Effect of hypoxia on natriuretic peptides and VEGF production in AC16 cells. gp70 blocking. Cell Culture AO391 (derived from human aorta) and CD314 (derived from human carotid artery) cell lines were a generous gift from Wendy Bauer (Tufts Molecular Cardiology Research Institute) and were grown in DMEM (CellGro) supplemented with 10% FBS. Subsequently, the oxidative burst was initiated by the addition of PMA (final concentration 100 nM) and the cells incubated for 120 min at 37°C. Cell proliferation was measured using the MTT method. This is a used Molecular Devices SpectraMax 340PC Microplate Spectrophotometer. 5 g/L-EDTA 0. SpectraMax i3x 和 SpectraMax iD5 微孔板读板机上使用 SoftMax Pro 软件中的预设方法进行测量(有关仪器特定设 置，请参见表 2 和表 3 )。在 SpectraMax i3x 和 SpectraMax iD5 读板机上进行了微孔板优化和读取高度调整，以确保最佳 的检测灵敏度和动态范围。 数据分析SpectraMax® Absorbance Microplate Readers can run both cuvette-based and microplate reader applications on the same instrument. Then the samples were incubated for 1 h with human IL6 detecting antibody and then with HRP conjugate for 40 min. Irene Tieleman, Ido PenandSimon VerhulstSubsequently, the oxidative burst was initiated by the addition of PMA (final concentration 100 nM) and the cells incubated for 120 min at 37°C. SpectraMax ® Plus 384 optics. The light features a slim profile to prevent. Not all manufacturers' microplates are the same with regard to design, materials, orSpectraMaxABS Plus,SpectraMaxM2,SpectraMaxM2e,SpectraMax M3,SpectraMaxM4,SpectraMaxM5,SpectraMaxM5e,SpectraMaxPlus 384 0200-7200 Multi-Mode ValidationPlate. 0 3. mums. Briefly, cells were seeded into a. All assays were done in triplicate with readings at 60-s intervals using a SpectraMax 340 multiwell plate reader (Molecular Devices, San Jose, CA, United States). The SpectraMax® 340 PC 384 Absorbance Microplate Reader from Molecular Devices provides the necessary tools for absorbance measurements in the visible range.